invincibility is automatic...

[Hilbert]

Whenever I boot up VVVVVV it atuomaticly turns INVINCIBILITY on. It bugs me when testing my levels.

edit: HAHA I also noticed if a crewmate is in a room and if they are happy and you die they turn sad until you respawn

[Bearboy]

happens to me sometimes. delete your vvv save data when it's off, and it'll fix the problem.
for who knows how long.

[FIQ]

Make sure to exit VVVVVV properly, and also make sure that VVVVVV is able to write to unlock.vvv. Try to alter other settings and see if they change. If they change but invincibility does stay on, there's something else that is the problem.

[blue626]

Maybe you forgot to turn off invincibility before quitting. I think the game remembers if you activated invincibility or not, after quitting.

[Vallu]

I guess it goes off if you play the game with invisiblity off and then save the game.

[Arthur]

I also got the same problem once. It just happens when you quit VVVVVV without using QUIT in the main menu, and it was booted with inv. on. Never touch that red button...